Amide-based molecules play a significant role in various scientific and industrial applications, including pharmaceuticals and agrochemicals, due to their structural and functional properties. This study focuses on the synthesis and evaluation of a novel amide, N-(2-bromoethyl)-4-nitrobenzamide, to enhance antimicrobial properties. The amide was synthesized via a condensation reaction between 4-nitrobenzoyl chloride and 2-bromoethylamine hydrobromide, using pyridine as a base. The structural characterization was conducted through ¹H and ¹³C NMR spectroscopy, confirming the successful formation of the amide bond. To improve the antibacterial efficacy, a supramolecular ensemble was formed by incorporating the synthesized amide with graphene oxide nanolayers, prepared using a modified Hummer method. The antibacterial activity of the amide and its ensemble was assessed against bacterial strains including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and  Klebsiella pneumoniae. Results showed that the graphene oxide enhanced the antimicrobial potency, with significantly lower minimum inhibitory concentrations (MICs) compared to the amide alone and the standard antibiotic ampicillin. The study demonstrates the potential of amide-graphene oxide composites for developing new antibacterial agents with improved activity.

doi.org/10.32737/0005-2531-2025-4-61-69