Biomimetic sensors based on semiconductor (Si), metal (Al) and smart material (TPhPFe3+OH/Al2O3) have been developed. It has been found that a biomimetic sensor, where a semiconductor (Si) is used as an electrode, exhibits high sensitivity, stability and reproducibility. The effect of temperature and the amount of smart material on the catalase activity of biomimetic sensors was studied. At the study of the Si-electrode for catalase activity, it was revealed that the amount of smart material and temperature do not affect the detection limit of hydrogen peroxide in an aqueous solution, the sensitivity threshold, which was 10-6 mass%. When detecting the catalase activity of the TPhPFe3+OH/Al2O3//Al biomimetic sensor, it was established that the sensitivity threshold was also 10-6 mass %.